Sequencing ultra-long DNA molecules with the Oxford Nanopore MinION
John M Urban, Jacob Bliss, Charles E Lawrence, Susan A Gerbi
Oxford Nanopore Technologies’ nanopore sequencing device, the MinION, holds the promise of sequencing ultra-long DNA fragments >100kb. An obstacle to realizing this promise is delivering ultra-long DNA molecules to the nanopores. We present our progress in developing cost-effective ways to overcome this obstacle and our resulting MinION data, including multiple reads >100kb.